nebnext ultra ii directional rna library prep kit (New England Biolabs)

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New England Biolabs nebnext ultra ii directional rna library prep kit
Nebnext Ultra Ii Directional Rna Library Prep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 8969 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnas/NEBNext+Ultra+II+Directional+RNA+Library+Prep+Kit+for+Illumina/pm41662857-79-12-20
Average 99 stars, based on 8969 article reviews

nebnext ultra ii directional rna library prep kit - by Bioz Stars, 2026-10

99/100 stars

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Article Title: Mechanistic insights into RNA chaperoning by Ro60 and La autoantigens.
Article Snippet: Cell lysates were cleared by spinning 50 min at 284,600 × g and applied to His Pur Ni-NTA resin (Thermo Scientific 88222) pre-equilibrated with buffer A.

Article Title: Mechanistic Insights into RNA Chaperoning by Ro60 and La Autoantigens
Article Snippet: For in vitro transcription, sequences corresponding to wild-type X. laevis pre-5S rRNA 35 , the mutant misfolded (stem mt 1 + 2) pre-5S rRNA 36 , the 64 nt minimal misfolded pre-5S rRNA 41 , the minimal misfolded pre-5S rRNA with 15 additional Us at the 3’ end, the human pre-5S RNA, the human U-tailed 5S rRNA, and the human misfolded pre-5S rRNA were cloned into the HindIII/XhoI sites of p2RZ (Addgene 27644) containing an upstream hammerhead ribozyme and a downstream HDV ribozyme 91 under control of a T7 promoter.

Synthesized:

Article Title: Phage-encoded small RNA hijacks host replication machinery to support the phage lytic cycle
Article Snippet: .. RNAs were synthesized using T7 RNA polymerase (25 units, New England Biolabs) in a 50 μL reaction containing 1 X T7 RNA polymerase buffer, 10 mM DTT, 20 units of recombinant RNase inhibitor, 500 μM of each NTP, and 300 ng of T7 promoter containing template DNA at 37 °C for 2 hours, followed by 10 min at 70 °C. ..

Article Title: Phage-encoded small RNA hijacks host replication machinery to support the phage lytic cycle.
Article Snippet: .. RNAs were synthesized using T7 RNA polymerase (25 units, New England Biolabs) in a 50 μL reaction containing 1 X T7 RNA polymerase buffer, 10 mM DTT, 20 units of recombinant RNase inhibitor, 500 μM of each NTP, and 300 ng of T7 promoter containing template DNA at 37 ◦ C for 2 hours, followed by 10 min at 70 ◦ C. Next, Turbo DNase was added, and the reactions were incubated at 37 ◦ C for 30 min. ..

Recombinant:

Purification:

Article Title: Synergistic degradation of circular RNAs by RNAseK and lysosome.
Article Snippet: .. Purified RNAs were then ligated by T4 RNA ligase 1 (NEB, M0204) overnight at 16◦C according to the manufacturer’s procedure. ..

Article Title: A scalable screening platform for discovering predominant miRNA target genes and druggable siRNA sequences
Article Snippet: Cells were seeded at 50–60% confluence the day prior to transfection using GeneJuice Transfection Reagent (Sigma-Aldrich, cat# 70967) or Lipofectamine 2000 Transfection Reagent (Thermo, cat# 11668019) according to the manufacturer’s instructions. .. Purified small RNAs were ligated to a 3’ adaptor (/5rApp/NNNNTGGAATTCTCGGGTGCCAAGG/3ddC/) using T4 RNA ligase 2, truncated KQ (NEB, cat# M0373L) in 20% PEG-8000. .. Following the removal of culture medium, cells were lysed with TRIzol (Thermo, cat# 15596018) according to the manufacturer’s instructions.

Article Title: Programmable initiation of mRNA translation by trans-RNA.
Article Snippet: Several approaches exist to silence genes, but few tools are available to activate individual mRNAs for translation inside cells.. Guiding ribosomes to specific start codons without altering the original sequence remains a formidable task.. Here we design capped trans-RNAs capable of directing ribosomes to specific initiation sites on individual mRNAs when the trans-cap is positioned near the target start codon.

Incubation:

cDNA Synthesis:

Article Title: Multi-omic phenotyping of MAPT V337M neurons reveals early changes in axonogenesis and tau phosphorylation
Article Snippet: RNA was harvested from day 7, day 14, and day 28 post-differentiation neurons using a Zymo microprep kit (Zymo Research, Cat No. R2062). .. The library was prepared by first depleting ribosomal RNA (New England BioLabs, Cat No. E7405L). cDNA synthesis was then performed on all remaining RNAs (New England BioLabs, Cat. No. E7765S). .. Paired-end (PE65) sequencing was performed at the Chan Zuckerberg Biohub and the UCSF Center for Advanced Technology.

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